An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation
Advances in genome engineering have resulted in the generation of numerous zebrafish biomat for sale mutant lines.A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH).Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-type and mutant siblings can be done b